This time, they expect to face a full and rested MLS team and a pretty good one at that.
DC United will have six days off since their last league game. Their next game is June 28th. The break is due to the World Cup.
As a result, the Rhinos expect DC United to start most or all of their starters in effort to keep them sharp. Normally, Open Cup games feature MLS backups facing the lower level teams.
"We know it'll be difficult to sit in and keep it at 0-0," Rhinos head coach Bob Lilley said. "We're gonna try and come out and create chances and hopefully work our way into the game. "
Not only is the United likely to send their best team to Sahlen's Stadium, it's a pretty good "best" team. DC is 7-4-4 and leading the Eastern Conference.
"It's going to be good to experience that and the pressure because we have nothing to lose," Rhinos goalkeeper John McCarthy said. "So we can just take it to them and see what happens."
Rochester hasn't beaten an MLS team in the Open Cup since 2005. The glory days of the 1999 championship seem long behind, but that doesn't mean more glory isn't in the future.
The game will kickoff at 7:05pm Tuesday night.